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作者采集了26名轮状病毒胃肠炎患儿(9～37个月,平均年龄19个月)发病前、发病期间和发病后的血标本共129份,在用ELISA分析时,用四种人IgG亚类特异的单克隆抗体检测了这些标本中的轮状病毒特异性IgG亚类抗体。在每一名患儿的大便标本中,用ELISA至少能检测到一次轮状病毒抗原。检查结果:在发病前,有13名患儿未查到IgG_1抗体,只有8名患儿查到IgG_3抗体。感染轮状病毒后,所有26名患儿的IgG_1和IgG_3抗体水平都升高。IgG_3抗体表现出早期增长的特点,在从粪便中查到轮状病毒抗原后1周内,其效价达到峰值,随后下
The authors collected a total of 129 blood samples from 26 children with rotavirus gastroenteritis (9-37 months, mean age 19 months) before, during, and after onset of disease. In the ELISA analysis, Human IgG subclass-specific monoclonal antibodies detected rotavirus-specific IgG subclass antibodies in these specimens. In each stool specimen from a child, at least one rotavirus antigen was detected by ELISA. Test results: Before onset, 13 children were not detected IgG_1 antibody, only 8 children found IgG_3 antibody. After infection with rotavirus, IgG1 and IgG3 antibody levels were elevated in all 26 infants. The IgG3 antibody showed early growth characteristics and its titer reached a peak within 1 week after the rotavirus antigen was found in the stool,