Preventive effect of Qianggan-Rongxian Decoction on rat liver fibrosis

来源 :World Journal of Gastroenterology | 被引量 : 0次 | 上传用户:cyx810625
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
AIM: To study the preventive effects of Qianggan-Rongxian Decoction on liver fibrosis induced by dimethylnitrosamine (DMN) in rats. METHODS: Male Wistar rats were randomly divided into hepatic fibrosis model group, control group and 3 treatment groups (12 rats in each group). Except for the normal control group, all the rats received 1% DMN (10 μL/kg body weight, i.p), 3 times a week for 4 wk. The rats in the 3 treatment groups including a high-dose DMN group (10 mL/kg), a medium-dose DMN group (7 mL/kg), and a low-dose DMN group (4 mL/kg) were daily gavaged with Qianggan-Rongxian Decoction, and the rats in the model and normal control groups were given saline vehicle. Enzyme-linked immunosorbent assay (ELISA) was used to determine the changes in serum hyaluronic acid (HA), laminin (LN), and type IV collagen levels. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured using routine laboratory methods. Pathologic changes, particularly fibrosis, were examined by hematoxylin and eosin (HE) and Sirius red staining. Hepatic stellate cells (HSC) were examined by transmission electron microscopy. RESULTS: Compared with the model control group, the serum levels of HA, LN, type Ⅳ collagen, ALT and AST were decreased markedly in the other groups after treatment with Qianggan-Rongxian Decoction, especially in the medium-dose DMN group (P < 0.05).Moreover, the area-density percentage of collagen fibrosis was lower in the Qianggan-Rongxian Decoction treatment groups than in the model group, and a more significant drop was observed in the medium-dose DMN group (P < 0.05). CONCLUSION: Qianggan-Rongxian Decoction can inhibit hepatic fibrosis due to chronic liver injury, delay the development of cirrhosis, and notably ameliorate liver function. It may be used as a safe and effective thera-peutic drug for patients with fibrosis.
其他文献
目前蛋白质组研究越来越受到科学工作者的关注 ,随着研究技术的不断完善和发展 ,蛋白质组在医学上的应用越来越广泛。本文概述了蛋白质组在临床医学中的研究进展 ,包括肝癌等
6月8日下午,北京保利2014春季艺术品拍卖会8天60个专场成功落下帷幕,总成交额27.8亿元,连续第12次在国内大型艺术品拍卖会中列成交额榜首。其中成交价(5577.5万元)最高的拍品
“三唑锡”是70年代发展起来的有机锡杀螨剂,主要用于防治敏感性和抗性的螨类。1987年南开大学元素所研制成功。现已由山东招远三联化工厂批量生产。4年中全国已有7个单位对
JP2006141346-A本品由香菇(shiitake mushroom)菌丝体的提取物组成,可有效地激活、诱导未成熟的神经元树突细胞成为成熟的具有低氧化应激反应的神经元树突细胞。用于药品或功
许多失眠病人对使用镇静催眠药有很强的顾虑,担心出现各种不良反应。病人的顾虑很正常,在开镇静催眠药处方前,医生有责任向病人讲清镇静催眠药物的适应症和禁忌症。而且, Ma
自然界充满有毒的物质。空气、水、食物这些普普通通的物质,如果我们未能正确利用或过分吸收,都将不可避免地引发中毒症状。如若人体内所含盐分增加到正常值的10倍,就会导致
目的探讨立体定向术(MIQ)联合化疗治疗PCNSL的效果分析。方法本次研究随机选取了2013年4月至2015年8月我院收治的50例明确病理后的PCNSL患者,随机分为数目相等的研究组和对照
管氏肿腿蜂Scleroderma guani Xiao et Wu作为钻蛀性害虫的天敌,已在我国部分地区应用,并收到显著效益。大量繁殖该蜂,则必须解决繁蜂的中间寄主问题。目前国内外已有些这方
本刊讯(记者韩阳)2006年底上海画报出版社悄然完成了社名的更改,正式更名为上海锦绣文章出版社,更名后的出版社打破原有图书出版格局,首先向时尚图文领域 Our correspondent
处方药是必须凭执业医师或执业助理医师处方才可调配、购买和使用的药品;非处方药是不需要凭医师处方即可自行判断、购买和使用的药品。非处方药在国外又称之为“可在柜台上