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皮下注射S180细胞建立动物实体瘤模型,将小鼠随机分为生理盐水对照组和猪软骨多糖治疗组,连续腹腔注射生理盐水或猪软骨多糖21d,MTT法测定脾细胞增殖能力、免疫荧光法检测CD40和CD40L的表达以及TUNEL法检测瘤细胞的凋亡;免疫组化SP法检测Fas、PCNA、CyclinD1和p21的表达。结果显示,猪软骨多糖能够促进脾淋巴细胞的转化并提高CD40和CD40L蛋白的表达,治疗组的Fas和p21的抗体表达较对照组显著升高,而PCNA和CyclinD1的表达则显著降低。提示猪软骨多糖能够活化免疫细胞,提高小鼠的免疫力,通过提高Fas和p21的表达水平和降低PCNA和Cy-clinD1的表达水平而诱导瘤细胞的凋亡。
Subcutaneous S180 cells were used to establish a solid animal tumor model. The mice were randomly divided into normal saline control group and porcine cartilage polysaccharide treatment group. Intraperitoneal injection of physiological saline or porcine cartilage polysaccharide was performed for 21 days. The proliferation of spleen cells was measured by MTT assay and immunofluorescence assay. The expression of CD40 and CD40L and the apoptosis of tumor cells were detected by TUNEL method. The expression of Fas, PCNA, CyclinD1 and p21 was detected by immunohistochemistry SP method. The results showed that porcine cartilage polysaccharide can promote the transformation of spleen lymphocytes and increase the expression of CD40 and CD40L proteins. The expression of Fas and p21 antibodies in the treatment group was significantly higher than that in the control group, while the expression of PCNA and CyclinD1 was significantly decreased. It was suggested that porcine cartilage polysaccharide can activate immune cells and increase the immunity of mice, and induce apoptosis of tumor cells by increasing the expression of Fas and p21 and decreasing the expression of PCNA and Cy-clinD1.