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目的了解安徽省哨点医院腹泻患儿中病毒性病原体感染状况和分子流行病学特征。方法收集安徽省两哨点医院2011年1月~2012年12月住院腹泻患儿粪便标本549份,采用酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)法检测轮状病毒(rotavirus,RV)抗原,采用聚合酶连锁反应(polymerase chain reaction,PCR)或反转录聚合酶连锁反应(reverse transcription-PCR,RT-PCR)检测RV、诺如病毒(norovirus,NoV)、星状病毒(astrovirus,AstV)和肠道腺病毒(adenovirus,AdV)并分型鉴定。结果 549份粪便标本中RV检出率为22.40%(123/549),NoV为12.02%(66/549),AstV为1.82%(10/549),AdV为1.28%(7/549),混合感染1.15%(4/549)。对RV G/P分型结果显示G9P[8]为主要流行株,NoV以GII-4为主要流行株。结论安徽哨点医院婴幼儿病毒性腹泻的病原以RV为主,秋冬季高发,存在混合感染。
Objective To understand the prevalence and molecular epidemiological characteristics of viral pathogens in children with diarrhea at sentinel hospital in Anhui Province. Methods 549 stool specimens of children with diarrhea in hospital from January 2011 to December 2012 in two sentinel hospitals of Anhui province were collected. The rotavirus (RV) was detected by enzyme linked immunosorbent assay (ELISA) Antigen was detected by polymerase chain reaction (PCR) or reverse transcription-PCR (RT-PCR). The results showed that RV, norovirus (NoV), astrovirus AstV) and enterovirus (adenovirus, AdV) and identified by typing. Results The detection rate of RV in 549 stool specimens was 22.40% (123/549), NoV was 12.02% (66/549), AstV was 1.82% (10/549), AdV was 1.28% (7/549) Infection was 1.15% (4/549). The results of RV G / P typing showed that G9P [8] was the main epidemic strain and NoV was the major epidemic strain of GII-4. Conclusions The pathogen of infantile viral diarrhea in Anhui sentinel hospital is mainly RV, with high incidence in autumn and winter, and there is a mixed infection.