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葡萄球菌蛋白A(SPA)是金黄色葡萄球菌细胞壁的一种蛋白成份,具有与人及多种哺乳动物血清中IgG类抗体Fc段结合的特性。国外自七十年代起对SPA广泛开展了研究;国内自1979年由张颖悟引进标准株以来也进行了大量研究。近年国内外学者应用SPA作为一种新的免疫学试剂,建立了许多敏感、特异、快速而简便的检测方法,本文仅就SPA在病毒学诊断中的应用综述如下。一、SPA主要性质 SPA分子是高度稳定的单一多肽链,分子量为42000。从N端开始有4个高度同源性片段,每个片段都具有与IgG Fc段结合的特性。SPA具有多结合片段的特性使SPA碘化
Staphylococcal protein A (SPA), a protein component of the cell wall of Staphylococcus aureus, has the property of binding to the Fc region of an IgG class antibody in human and various mammalian sera. Since the 1970s, foreign countries have carried out extensive research on SPA. Since 1979, the introduction of standard strains by Zhang Yingwu has also been conducted a large number of studies. In recent years, domestic and foreign scholars using SPA as a new immunological reagents, the establishment of a number of sensitive, specific, rapid and easy detection methods, this article only on the SPA in the diagnosis of virology are summarized below. First, the main properties of SPA SPA molecules are highly stable single polypeptide chain, the molecular weight of 42,000. There are four highly homologous fragments starting from the N-terminus, each of which has the property of binding to the IgG Fc fragment. SPA has the characteristics of multi-binding fragment SPA iodinated