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目的:建立检测原芹菜素(protoapigenone)在人与鼠血浆药物浓度的高效液相色谱法(HPLC),测定原芹菜素在人与鼠血浆的蛋白结合率。方法:采用平衡透析法,结合HPLC测定原芹菜素在人与鼠血浆的蛋白结合率。结果:原芹菜素人血药透析袋内外平衡时间约为10h,在低、中、高(200,500,1000μg.L-1)质量浓度下,其血浆蛋白结合率为(90.5±1.2)%,(89.6±1.7)%,(91.4±1.4)%;鼠血药透析袋内外平衡时间约为6h,在低、中、高(200,500,1000μg.L-1)质量浓度下,鼠血浆蛋白结合率为(80.7±2.2)%,(81.1±1.8)%,(81.9±1.3)%。结论:原芹菜素在人、鼠血浆蛋白结合率之间差异有显著性(P<0.01),但同一样品低、中、高浓度间,蛋白结合率差异无显著性(P>0.05)。原芹菜素与人、鼠血浆蛋白具有较强的结合。
OBJECTIVE: To establish a high performance liquid chromatography (HPLC) for the determination of protoapigenone in human and mouse plasma and to determine the protein binding rate of procainin in human and mouse plasma. Methods: The protein binding rate of apigenin in human and mouse plasma was determined by equilibrium dialysis and HPLC. Results: The internal and external equilibration time of prochiurin hemodialysis dialysis bag was about 10h, and the plasma protein binding rate was (90.5 ± 1.2)%, (89.6 ± 0.18)% at low, medium and high concentrations ± 1.7%, and (91.4 ± 1.4)%, respectively. The internal and external equilibration time of rat hemodialysis dialysis bag was about 6 hours. The plasma protein binding rate of rats was ( 80.7 ± 2.2)%, (81.1 ± 1.8)%, (81.9 ± 1.3)%. Conclusion: There was a significant difference in the plasma protein binding rate between progestin and human (P <0.01). However, there was no significant difference in the protein binding rate of the same sample between low, medium and high concentrations (P> 0.05). Proendin and human, mouse plasma protein has a strong combination.