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目的 探讨单、双标记技术对T细胞亚群测定的影响。方法 采用荧光抗体双标记、流式细胞仪对正常人外周血细胞进行了测定。结果 CD3 + CD4 + 与CD4 + 细胞比较 ,差异无显著性意义 ,CD3 + CD8+ 与CD8+ 比较 ,有显著性差异 (P <0 .0 5 ) ,CD3 + CD4 + /CD3 + CD8+ 与CD4 + /CD8+ 比较 ,差异有显著性意义 (P <0 .0 5 )。结论 检测T细胞亚群应采用双标记技术。
Objective To investigate the effect of single and double labeling on the determination of T cell subsets. Methods Fluorescent antibody double labeling, flow cytometry of normal human peripheral blood cells were measured. Results There was no significant difference between CD3 + CD4 + and CD4 + cells. CD3 + CD8 + and CD8 + had significant difference (P <0.05), CD3 + CD4 + / CD3 + CD8 + and CD4 + / CD8 + The difference was significant (P <0.05). Conclusion T-cell subpopulations should be double-labeled.