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大蒜是我国重要蔬菜之一,它以蒜瓣做繁殖材料,繁殖系数很低,仅是播种量的几倍或十几倍,难于满足生产发展的需要。植物离体组织培养的发展,已引起人们的注意,用之做为加速植物繁殖和改进品种的手段。在兰、草莓、马铃薯等园艺植物方面早已获得成功,并在生产中运用。1973,Harva＇nck氏等以大蒜叶获得愈伤组织并诱导出幼苗,小鳞茎。1676,Kohr氏又从蒜茎诱导出愈伤组织,幼苗,1980,周云罗等以贮藏叶愈伤组织诱导出幼苗。我们试图探索以大蒜鳞茎切割与嫩叶培养诱导愈伤组织进而分化成苗,以提高大蒜的无性繁殖系数。
Garlic is one of the most important vegetables in our country. It is made of garlic cloves and its propagation coefficient is very low, which is only several times or ten times of that of seeding. It is difficult to meet the needs of production and development. The development of plant tissue culture in vitro has drawn the attention of people as a means of accelerating plant propagation and improving varieties. Success has been achieved in horticultural plants such as blueberries, strawberries and potatoes, and is being used in production. In 1973, Harva’nck’s et al obtained callus from garlic leaves and induced seedlings and bulblets. 1676, Kohr’s again from the garlic stem callus induction, seedlings, 1980, Zhou Yunluo callus induction of seedlings with storage leaves. We tried to explore garlic bulb cutting and young leaves cultured callus induction and differentiation into seedlings in order to improve the clonal coefficient of garlic.