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利用EMS诱变粳稻品种武运粳7号获得一个新窄叶突变体nal10,该突变体在苗期表现出极窄叶和弱小的特征,在生殖期表现出矮化、窄叶、畸颖和不育的表型。组织解剖学分析表明,nal10的叶片窄化可能是由于叶片大脉和小脉数量减少造成的。遗传分析表明,该窄叶表型受一对隐性核基因控制。利用nal10与台中本地一号(TN1)构建的F2群体,通过混合分离法,在第1染色体上找到3个紧密连锁的SSR标记(RM1287、RM562和RM5638)。进一步利用新发展的分子标记,最终将该基因定位在STS标记PK83和PK78之间的55.2kb范围内,该区间共有8个开放阅读框。RT-PCR分析表明,NAL10的突变能够造成生长素生物合成、信号转导和运输基因转录水平的下降,因此NAL10是一个与生长素相关的窄叶新基因。这些结果为进一步克隆该基因、丰富水稻叶发育的遗传调控网络打下了基础。
A new narrow-leaf mutant, nal10, was obtained by EMS mutagenesis of japonica rice Wuyunjing 7. The mutant showed extremely narrow leaves and weak features at seedling stage and showed dwarf, narrow leaf, Sterile phenotype. Tissue anatomy analysis showed that the narrowing of nal10 leaves may be due to the reduction of leaf veins and veins. Genetic analysis shows that the narrow-leaf phenotype is controlled by a pair of recessive nuclear genes. Three closely linked SSR markers (RM1287, RM562 and RM5638) were found on chromosome 1 using the F2 population constructed by nal10 and TN1 locality in Taichung. Further utilizing newly developed molecular markers, the gene was finally located within 55.2 kb of the STS marker PK83 and PK78 with a total of eight open reading frames. RT-PCR analysis showed that NAL10 mutation caused a decrease of auxin biosynthesis, signal transduction and transport of gene transcription, so NAL10 is a auxin-associated narrow-leaf gene. These results lay the foundation for further cloning of this gene and enriching the genetic regulation network of rice leaf development.