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以一年生甘草移栽苗为实验材料,设置5个锰离子浓度水平,分别为0、1.81、18.1、36.2和54.3 mg·L-1(Mn SO4·H2O),利用实时荧光定量PCR和高效液相色谱方法对甘草鲨稀合成酶1(SQS1)基因相对表达量和甘草酸含量进行测定,从而探讨锰处理对甘草SQS1基因表达量与甘草酸积累的影响。结果表明随着锰处理浓度的增加,甘草SQS1基因的相对表达量和甘草酸含量均呈现先升高后下降的趋势,且两者之间存在极显著正相关(P<0.01,r=0.737)。SQS1基因表达量在18.1 mg·L-1浓度处理时达到最大值为7.90,分别是对照(0 mg·L-1)、1.81、36.2和54.3 mg·L-1处理的1.75、1.37、1.37和2.33倍,且差异极显著(P<0.01)。甘草酸含量在1.81和18.1 mg·L-1浓度处理时达到最大值,且两者之间差异不显著(P>0.05),与其他3个处理间存在极显著差异(P<0.01)。本研究说明适量浓度的锰处理对甘草SQS1基因的表达和甘草酸的积累具有一定的促进作用。
Five year-old licorice transplanted seedlings were used as experimental materials, and five manganese ion concentrations were set as 0,1.81,18.1,36.2 and 54.3 mg · L-1 (Mn SO4 · H2O) respectively. Real-time fluorescence quantitative PCR and high performance liquid chromatography Chromatographic methods were used to determine the relative gene expression of SQS1 and glycyrrhizic acid content in Glycyrrhiza shark so as to explore the effect of manganese treatment on the expression of SQS1 gene in Glycyrrhiza uralensis and glycyrrhizin accumulation. The results showed that with the increase of manganese concentration, the expression level of SQS1 and glycyrrhizic acid content of licorice tended to first increase and then decrease, and there was a significant positive correlation between them (P <0.01, r = 0.737) . SQS1 gene expression reached the maximum of 7.90 at the concentration of 18.1 mg · L-1, which were respectively 1.75, 1.37, 1.37 and 1.81, 36.2 and 54.3 mg · L-1 of control (0 mg · L-1) 2.33-fold, and the difference was significant (P <0.01). Glycyrrhizic acid reached the maximum value at the concentrations of 1.81 and 18.1 mg · L-1, and there was no significant difference between the two (P> 0.05), which was significantly different from the other three treatments (P <0.01). This study shows that the appropriate concentration of manganese treatment of licorice SQS1 gene expression and glycyrrhizic acid accumulation has a certain role in promoting.