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从人血清、血浆、乳汁等体液中萃取出游离脂肪酸与酯化态脂肪酸混合物,经薄层层析分离、甲酯化后,以十五(烷)酸作内标进行GC/MS定量测定,获得游离脂肪酸谱(Free Fatty Acid Profiles)。
The mixture of free fatty acids and esterified fatty acids was extracted from body fluids such as human serum, plasma and milk and separated by thin layer chromatography. After methyl esterification, GC / MS quantitative determination was performed with pentadecane acid as internal standard. Free Fatty Acid Profiles were obtained.