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目的:观察核因子-κB(NF-κB)在油酸致大鼠急性肺损伤时肺组织中的表达变化,探讨葛根素对急性肺损伤的保护作用及其可能机制。方法:24只大鼠随机分为3组:对照组(Control)、油酸组(OA)、葛根素预处理组(Pue)。Control组大鼠尾静脉注射生理盐水0.1 ml·kg-1;OA组大鼠尾静脉注射油酸0.1 ml·kg-1;Pue组大鼠在注射油酸前30 min腹腔注射葛根素30 mg·kg-1。光镜下观察肺组织病理形态学改变,免疫组织化学染色技术观察NF-κB在肺组织中的表达变化。结果:Pue组较OA组肺组织损伤情况明显减轻。OA组肺组织NF-κB的阳性表达(0.39±0.07)与Conrtol组(0.12±0.04)相比显著增加(P<0.05);应用葛根素后肺组织NF-κB的阳性表达(0.24±0.05)明显减少,但仍高于Conrtol组(P<0.05)。结论:葛根素可能通过抑制NF-κB的表达减轻急性肺损伤时肺组织的炎症反应。
Objective: To observe the expression of nuclear factor-κB (NF-κB) in the lung tissue of acute lung injury induced by oleic acid in rats and explore the protective effect of puerarin on acute lung injury and its possible mechanism. Methods: Twenty-four rats were randomly divided into three groups: control group, oleic acid group and Puerarin preconditioning group. The tail vein of Control group was injected with 0.1 ml · kg-1 of physiological saline; the tail vein of OA group was injected with 0.1 ml · kg-1 oleic acid; Puerarin 30 mg · kg-1. The pathological changes of lung tissue were observed under light microscope. The expression of NF-κB in lung tissue was observed by immunohistochemical staining. Results: The lung injury in Pue group was significantly lower than that in OA group. The positive expression of NF-κB in lung tissue of OA group (0.39 ± 0.07) was significantly higher than that of Conrtol group (0.12 ± 0.04) (P <0.05) Significantly decreased, but still higher than Conrtol group (P <0.05). Conclusion: Puerarin may reduce the inflammatory reaction of lung tissue in acute lung injury by inhibiting the expression of NF-κB.