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为探讨miRNA-467b在实验性自身免疫性脑脊髓膜炎(experimental autoimmune encephalomyelitis,EAE)中对炎性T细胞浸润的影响,我们首先采用real-time PCR及FACS方法检测EAE小鼠脾脏单个核细胞中CXCR3、CCR5、CCR2、CCR6的mRNA和蛋白表达水平,并在MOG反应性T细胞中过表达miRNA-467b,FACS方法检测趋化因子受体CXCR3、CCR6的表达变化,并进一步通过Boyden小室(transwell实验)的方法,体外观察miRNA-467b对细胞迁移的影响。结果发现,EAE小鼠脾脏中CCR5、CCR2、CCR6的转录及蛋白表达水平显著上升,在MOG特异性的T细胞中过表达miRNA-467b后,可降低Th1表面趋化因子受体的表达,减少Th1向transwell板下室中的迁移,提示miRNA-467b可以通过影响细胞的迁移进而调节EAE的疾病进程。
To investigate the effect of miRNA-467b on the infiltration of inflammatory T cells in experimental autoimmune encephalomyelitis (EAE), we first detected the expression levels of splenic mononuclear cells in EAE mice by real-time PCR and FACS The expression of CXCR3, CCR5, CCR2 and CCR6 mRNA and protein in MOG reactive T cells was overexpressed and the expression of chemokine receptors CXCR3 and CCR6 were detected by FACS and further detected by Boyden chamber transwell experiments) method, in vitro observation miRNA-467b on cell migration. The results showed that the transcription and protein expression of CCR5, CCR2 and CCR6 in the spleens of EAE mice increased significantly. After overexpression of miRNA-467b in MOG-specific T cells, the expression of Th1 surface chemokine receptors was decreased and decreased The migration of Th1 into the transwell chamber suggests that miRNA-467b regulates the progression of EAE by affecting cell migration.