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目的:观察抗肝纤饮对实验性肝纤维化大鼠的防治作用,并探讨其作用机制。方法:48只雄性 SD 大鼠被随机分为正常组、模型组、抗肝纤饮及秋水仙碱组,采用 ih40% CCl_4花生油溶液诱导大鼠肝纤维化模型。造模同时,各组分别灌胃给予抗肝纤饮、秋水仙碱及0.9%氯化钠溶液治疗。于11周末检测大鼠血清透明质酸(HA)、Ⅳ型胶原(CⅣ)、层粘蛋白(LN)、血清转化生长因子β_1(TGF-β_1)的水平,检测肝组织 TGF-β_1阳性面积及 TGF-β_1 mRNA 的表达水平。结果:与模型组比较,抗肝纤饮组血清 HA、CⅣ及 TGF-β_1水平显著下降(P<0.01或 P<0.05),肝组织内 TGF-β_1阳性面积及 TGF-β_1 mRNA 的含量明显下降(P<0.01或 P<0.05)。结论:抗肝纤饮对实验性肝纤维化大鼠有较好的防治作用,其作用机制与降低血清及肝组织内 TGF-β_1有关。
Objective: To observe the preventive and therapeutic effects of Kangganxiu Yin on experimental hepatic fibrosis in rats and to explore its mechanism of action. METHODS: Forty-eight male SD rats were randomly divided into normal group, model group, anti-liver fiber drink and colchicine group. Rat liver fibrosis model was induced by ih40% CCl_4 peanut oil solution. At the same time, each group was given intragastric administration of anti-liver fiber drink, colchicine and 0.9% sodium chloride solution. At the end of the 11th week, serum levels of HA, CIV, LN and TGF-β_1 were measured and the positive area of TGF-β_1 in liver tissue was detected. Expression level of TGF-β_1 mRNA. Results:Compared with the model group, serum HA, CIV and TGF-β_1 levels in the anti-hepatic fibrosis group decreased significantly (P<0.01 or P<0.05). TGF-β_1 positive area and TGF-β_1 mRNA content in the liver tissue decreased significantly. (P<0.01 or P<0.05). Conclusion: Anti-Liangxianshui has better preventive and therapeutic effects on experimental hepatic fibrosis in rats. Its mechanism of action is related to the reduction of serum and hepatic tissue TGF-β_1.