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目的 探讨乳腺珠蛋白 (hMAM )mRNA在乳腺癌患者外周血中的表达及临床意义。方法 采用逆转录 聚合酶链方法检测 5 6例乳腺癌、8例乳腺增生和 8例正常乳腺者外周血中hMAMmRNA的表达 ,并比较了hMAMmRNA表达与乳腺癌生物学特性的关系 ,术后早期化疗对hMAMmRNA表达的影响。 结果 5 6例乳腺癌患者外周血中hMAMmRNA表达阳性率为 30 4 % (17/ 5 6 ) ,8例慢性乳腺增生者和 8例正常乳腺者hMAMmRNA表达均为阴性 ,2组hMAMmRNA表达差异有非常显著性意义 (χ2 =19 76 6 ,P <0 0 1)。hMAMmRNA的表达与患者年龄、原发癌灶大小 ,临床分期差异无显著性意义 (χ2 =1 2 5 6 ,P >0 0 5 )。 17例hMAMmRNA表达阳性者经术后早期短程化疗后 ,转阴率为4 1 2 % (7/ 17) ,手术前hMAMmRNA表达阴性者无一例表达阳性。 结论 hMAMmRNA特异表达于乳腺癌患者外周血 ,有可能成为诊断乳腺癌和判断预后的一个独立指标。
Objective To investigate the expression of mammary gland globin (hMAM) mRNA in peripheral blood of breast cancer patients and its clinical significance. Methods The expression of hMAM mRNA in peripheral blood of 56 cases of breast cancer, 8 cases of hyperplasia of mammary gland and 8 cases of normal breast tissue were detected by reverse transcription polymerase chain reaction. The relationship between hMAM mRNA expression and the biological characteristics of breast cancer was compared. Early postoperative chemotherapy On hMAM mRNA expression. Results The positive rate of hMAM mRNA expression in peripheral blood of 5 6 breast cancer patients was 30 4% (17/56), hMAM mRNA expression was negative in 8 cases of chronic hyperplasia of mammary gland and 8 cases of normal breast tissue, hMAM mRNA expression in 2 groups was significantly different Significant significance (χ2 = 19766, P <0 0 1). There was no significant difference between hMAM mRNA expression and patient’s age, primary tumor size and clinical stage (χ2 = 12.525, P> 0.05). In the 17 cases with positive hMAM mRNA expression, the rate of negative conversion was 41.2% (7/17) after the early short-term postoperative chemotherapy. No positive expression of hMAM mRNA was found in all 17 cases. Conclusion The specific expression of hMAM mRNA in the peripheral blood of patients with breast cancer may become an independent indicator of breast cancer diagnosis and prognosis.