论文部分内容阅读
AIM To investigate the apoptosis in gastric cancer cellsinduced by paclitaxel,and the relation between this apoptosisand expression of Bcl-2 and Bax.METHODS:In in vitro experiments,MTT assay was usedto determine the cell growth inhibitory rate.Transmissionelectron microscope and TUNEL staining method were usedto quantitatively and qualitively detect the apoptosis statusof gastric cancer cell line SGC-7901 before and after thepaclitaxel treatment.Immunohistochemical staining was usedto detect the expression of apoptosis-regulated gene Bcl-2and Bax.RESULTS:Paclitaxel inhibited the growth of gastric cancercell line SGC-7901 in a dose-and time-dependent manner.Paclitaxel induced SGC-7901 cells to undergo apoptosis withtypically apoptotic characteristics,including morphologicalchanges of chromatin condensation,chromatin crescentformation,nucleus fragmentation and apoptotic bodyformation.Paclitaxel could reduce the expression ofapoptosis-regulated gene Bcl-2,and improve the expressionof apoptosis-regulated gene Bax.CONCLUSION:Paclitaxel is able to induce the apoptosis ingastric cancer.This apoptosis may be mediated by down-expression of apoptosis-regulated gene Bcl-2 and up-expression of apoptosis-regulated gene Bax.
AIM To investigate the apoptosis in gastric cancer cells induced by paclitaxel, and the relation between this apoptosis and expression of Bcl-2 and Bax. METHODS: In in vitro experiments, MTT assay was used to determine the cell growth inhibitory rate. Transmission electron microscope and TUNEL staining method were used to quantitatively and qualitively detect the apoptosis status of gastric cancer cell line SGC-7901 before and after the paclitaxel treatment. Immunohistochemical staining was used to detect the expression of apoptosis-regulated gene Bcl-2 and Bax. RESULTS: Paclitaxel inhibited the growth of gastric cancer cell line SGC -7901 in a dose-and time-dependent manner. Paclitaxel induced SGC-7901 cells to undergo apoptosis with tyrosinase, morphological changes of chromatin condensation, chromatin crescentformation, nucleus fragmentation and apoptotic body formation. Paclitaxel could reduce the expression of apoptosis-regulated gene Bcl -2, and improve the expressionof apop tosis-regulated gene Bax.CONCLUSION: Paclitaxel is able to induce the apoptosis-ingastric cancer. This apoptosis may be mediated by down-expression of apoptosis-regulated gene Bcl-2 and up-expression of apoptosis-regulated gene Bax.