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目的:探讨VNTR(variable number of tandem repeat)技术在吉林省结核分枝杆菌基因分型中的应用。方法:初步选取15个分型效果较好的VNTR基因位点,采用聚合酶链反应(PCR)和琼脂糖凝胶电泳,并应用BioNumerics(Version3.0)软件进行处理,建立检测结核分枝杆菌DNA指纹多态性的方法,分析耐药结核分枝杆菌的DNA多态性。结果:共对320株结核分枝杆菌的15个VNTR位点进行了检测,根据这些菌株的指纹多态性特征,共分成26个型别,其中大部分属于一个型(Ⅰ型),占82.5%(264/320),其他菌株呈散在分布。结论:资料分析表明,吉林省结核分枝杆菌的传播似以Ⅰ型菌株为主,应加强此型菌株流行的监控。
Objective: To investigate the application of VNTR (variable number of tandem repeat) technology in the genotyping of Mycobacterium tuberculosis in Jilin province. Methods: Fifteen VNTR loci with good typing were selected initially, and PCR and agarose gel electrophoresis were performed. The VNTR loci were detected by BioNumerics (Version3) DNA fingerprinting polymorphism analysis of DNA polymorphism in drug-resistant Mycobacterium tuberculosis. Results: A total of 15 VNTR loci were detected in 320 strains of Mycobacterium tuberculosis. According to the fingerprinting polymorphism of these strains, they were divided into 26 types, most of which belonged to one type (type I), accounting for 82.5 % (264/320), other strains were scattered. Conclusion: The data analysis shows that the transmission of Mycobacterium tuberculosis in Jilin Province is mainly type Ⅰ strain, and the epidemic surveillance of this type of strain should be strengthened.