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文章报道了代谢性酸中毒时大鼠肾组织两种钠离子依赖的枸橼酸膜转运蛋白mRNA表达量的变化。给雌性Wistar大鼠喂含 0 2 8mol/LNH4Cl饮用水诱导产生代谢性酸中毒。喂酸后分别于 1、3、7d处死大鼠 ,测定血浆HCO 3浓度的变化。以Northern杂交方法 ,用钠离子依赖的枸橼酸膜转运蛋白 1(SDCT1)探针及钠离子依赖的枸橼酸膜转运蛋白 2 (SDCT2 )探针 ,分别检测肾皮质枸橼酸转运蛋白mRNA的表达变化。结果显示 ,喂酸后第 1天大鼠血浆内HCO 3浓度明显下降 ,与对照组比较差异显著 (P <0 0 1) ,但Northern杂交结果显示肾小管上皮细胞两侧的枸橼酸转运蛋白mRNA表达量无明显变化。第 3天 ,喂酸组大鼠血浆HCO 3浓度有所回升 ,但仍显著低于对照组 (P <0 0 1) ,肾小管上皮细胞刷状缘侧和基底侧膜的枸橼酸转运蛋白mRNA表达量均明显增加。第 7天喂酸组大鼠血浆HCO 3浓度继续回升 ,与对照组比较无显著差异 (P >0 0 5 ) ,肾小管上皮细胞两侧的枸橼酸转运蛋白mR NA表达仍明显增强。结果提示 ,在代谢性酸中毒时 ,大鼠肾小管上皮细胞两侧钠离子依赖的枸橼酸膜转运蛋白mRNA表达量增加 ,肾脏对枸橼酸的重吸收能力可能增强。
The paper reports the changes of two sodium ion-dependent citrate membrane transporter mRNA expressions in rat renal tissue during metabolic acidosis. Female Wistar rats were fed with 0 28 mol / L NH4Cl drinking water to induce metabolic acidosis. After fed with acid, rats were sacrificed at 1, 3 and 7 days respectively to determine the change of plasma HCO3 concentration. Northern blotting was used to detect the expression of citrate transporter 1 (SDCT1) probe and sodium ion-dependent citrate membrane transporter 2 (SDCT2) probe in renal cortex Changes in expression. The results showed that the concentration of HCO 3 in the plasma of the rats on the first day after feeding was significantly lower than that of the control group (P <0.01), but the results of Northern blot showed that both the citrate transporter No significant change in mRNA expression. On the third day, the concentration of HCO 3 in the hippocampus of rats fed with acid increased, but still significantly lower than that in the control group (P <0.01), citrate transporter of brush border and basolateral membrane of tubular epithelial cells mRNA expression increased significantly. On the 7th day, the concentration of HCO3 in the rats fed with acid continued to rise, which showed no significant difference compared with the control group (P> 0.05). The expression of citrate transport protein mR NA on both sides of renal tubular epithelial cells was still significantly increased. The results suggest that in metabolic acidosis, the expression of sodium ion-dependent citrate membrane transporter mRNA on both sides of rat renal tubular epithelial cells increased, renal reabsorption of citric acid may be enhanced.