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我们制备了高效价降钙素基因相关肽(CGRP)的免抗血清,现报道如下。1材料和方法1.1抗血清的制备将CGRP(军事医学科学院基础研究所提供)与牛血清白蛋白(BAS)偶联。称2mgCGRP和3mgBAS溶于预冷的0.lmmol/L、pH7.4的PBS250pl中,然后将预冷的50ml几成二醒400…,缓慢滴入
We prepared antiserum against high-potency calcitonin gene-related peptide (CGRP) and are reported below. 1 Materials and methods 1.1 Preparation of antisera CGRP (provided by Basic Research Institute of Military Academy of Medical Sciences) was coupled with bovine serum albumin (BAS). Weigh 2mgCGRP and 3mgBAS in precooled 0. lmmol / L, pH7.4 PBS250pl, and then the precooling 50ml several into two awake 400 ..., slowly dropping