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目的探讨高危型人乳头状瘤病毒(HR-HPV)合并人巨细胞病毒(CMV)、人疱疹病毒4型(EBV)、解脲脲原体(UU)感染在宫颈上皮内瘤变中的作用,并分析年龄与宫颈上皮内瘤变的相关性。方法选取2015年1-12月该院收治的宫颈高级别病变(HSIL)患者46例、低级别病变(LSIL)患者42例、未明确意义的非典型鳞状上皮细胞不排除病变(ASCUS)患者41例和正常对照(NILM)患者107例为研究对象。通过流式荧光杂交和实时荧光定量基因扩增法分别检测HR-HPV、CMV、EBV、UU感染情况。并通过SPSS 17.0软件运用χ~2检验和多项Logistic回归分析其感染状态与宫颈病变的相关性。结果UU感染率在各组中均为最高,其次是CMV,EBV感染率最低。UU、CMV感染及年龄在各病变组间差异有统计学意义(P<0.01);两种及以上微生物合并HR-HPV感染在各组间差异有统计学意义(P<0.01)。结论 CMV、EBV、UU合并HR-HPV感染分别与宫颈上皮内瘤变呈正相关,随年龄增大,病变风险增大。且UU更容易合并HR-HPV感染。HR-HPV及微生物联合检测在宫颈癌的预防治疗中具有重要作用。
Objective To investigate the role of high risk human papilloma virus (HR-HPV) combined with human cytomegalovirus (CMV), human herpesvirus type 4 (EBV) and ureaplasma urealyticum (UU) infection in cervical intraepithelial neoplasia , And analyze the correlation between age and cervical intraepithelial neoplasia. Methods Forty-six patients with high grade cervical lesions (HSIL), 42 patients with low grade lesions (LSIL) admitted to our hospital from January to December 2015 were enrolled in this study. Patients with unspecified atypical squamous cell carcinoma (ASCUS) 41 cases and normal control (NILM) patients 107 cases of the study. HR-HPV, CMV, EBV and UU infection were detected by flow cytometry and real-time fluorescence quantitative gene amplification. And SPSS 17.0 software using χ ~ 2 test and multiple logistic regression analysis of the relationship between infection status and cervical lesions. Results The prevalence of UU was the highest among all groups, followed by CMV, with the lowest EBV infection rate. UU, CMV infection and age were significantly different between the two groups (P <0.01). There was significant difference between two groups (P <0.01). Conclusions CMV, EBV and UU combined with HR-HPV infection have a positive correlation with cervical intraepithelial neoplasia respectively. As the age increases, the risk of the disease increases. And UU more likely to incorporate HR-HPV infection. Combined detection of HR-HPV and microorganisms plays an important role in the prevention and treatment of cervical cancer.