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目的从宽体金线蛭中分离纯化抗凝血活性成分。方法以抗凝血活性为筛选指标,以Sephadex G50、DEAE Cellulose-52、Sephadex LH-20等凝胶进行分离,利用HPLC进行纯化。结果从宽体金线蛭加热提取液中分离纯化得到一个肽类物质。结论宽体金线蛭中存在热稳定的抗凝血肽类物质。
OBJECTIVE To isolate and purify anticoagulant active ingredients from Hirudo broadleaf. Methods The anticoagulant activity was used as a screening index and separated by gel filtration on Sephadex G50, DEAE Cellulose-52, Sephadex LH-20 and purified by HPLC. Results A peptide was isolated and purified from Hirudo hamachii extract. Conclusion There are thermostable anticoagulant peptides in Hirudo nipponense.