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水稻白叶枯病是水稻生产中最严重病害之一。前期鉴定发掘的水稻资源材料Y238对白叶枯病具有广谱抗性,其衍生的以IR24为遗传背景的基因导入系CBB30Ⅰ保持了广谱抗性。利用抑制消减杂交(suppression subtractive hybridization,SSH)技术构建CBB30Ⅰ与感病品种IR24间的差异表达cD-NA文库,RT-PCR分析抗病相关基因表达情况。经反向Northern blot杂交检测、测序和GenBank中BLAST比对,结果共获得29个独立的差异表达cD-NA克隆,其中有3个功能未知基因。半定量RT-PCR进一步验证了抗感病品种间抗性相关基因的差异表达。根据MIPS(Munich Information Center forProtein Sequences)功能分类系统推测,这些差异表达基因可能参与了CBB30Ⅰ对病原菌的防卫反应、信号传导和蛋白合成与修饰等一些重要的生物学过程。
Rice bacterial leaf blight is one of the most serious diseases in rice production. Y238, a rice resource material previously identified and identified, has broad-spectrum resistance to bacterial leaf blight. Its derivative gene CBB30Ⅰ, which inherits the genetic background of IR24, has maintained broad-spectrum resistance. The cD-NA library was constructed by using suppression subtractive hybridization (SSH) technique to construct CBD30Ⅰand susceptible cultivar IR24, and the expression of disease-related genes was analyzed by RT-PCR. After reverse Northern blot hybridization, sequencing and BLAST alignment in GenBank, a total of 29 independent differentially expressed cD-NA clones were obtained, of which 3 function unknown genes. Semi-quantitative RT-PCR further validated the differential expression of resistance-related genes among susceptible susceptible cultivars. According to the functional classification system of MIPS (Munich Information Center for Protein Sequences), these differentially expressed genes may be involved in some important biological processes of CBB30I, such as defense response to pathogens, signal transduction and protein synthesis and modification.