目的:利用在线的方式,建立对3个属的新鲜黄芩样品中8种成分(褪黑素、5-羟色胺、吲哚-3-乙酸、金丝桃素、黄芩苷、黄芩素、汉黄芩苷、野黄芩苷)的液相色谱-质谱联用测定方法。方法:植物样品的匀浆与适量填料混合后,被填入相应的样品仓,通过杂质去除,目标物质富集洗脱等步骤,用于成分测定。结果:测定黄芩样品中8种成分,方法的检出限及定量限条件的回收率均大于90%,重复性及稳定性相对标准偏差不大于3.1%。结论:可以用于对黄芩原料中功效成分的评价。 OBJECTIVE: To establish an online method for the determination of 8 components of melatonin (melatonin, serotonin, indole-3-acetic acid, hypericin, baicalin, baicalein, , Wild baicalin) by liquid chromatography - mass spectrometry method. Methods: The homogenates of plant samples were mixed with the appropriate amount of filler, filled into the corresponding sample chamber, through the steps of impurity removal, enrichment and elution of the target substance for the determination of composition. Results: The detection limits and the recoveries of the limit of quantification were all above 90%. The relative standard deviations of repeatability and stability were no more than 3.1%. Conclusion: It can be used to evaluate the active ingredients in Radix Scutellariae.