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３５５例肺组织标本，其中正常组织１０例，单纯支气管粘膜上皮增生１１例，不典型增生１５例，原位癌１３例，浸润癌３０６例，用抗ｐ５３单抗Ｄｏ－７，进行免疫组化Ｓ－Ｐ法染色研究。结果发现：①正常支气管粘膜上皮和单纯增生上皮内ｐ５３蛋白染色呈阴性；②ｐ５３蛋白过量表达可见于４０．０％（６／１５）不典型增生，４６．２％（６／１３）的原位癌和５２．９％（１６２／３０６）的浸润癌；③ｐ５３阳性的组织增殖细胞核抗原（ＰＣＮＡ）频率明显高于ｐ５３阴性组织；④ｐ５３蛋白过量表达与患者临床分期呈正相关和生存时间呈明显负相关，但与肺癌组织学类型、分级无关。提示ｐ５３基因蛋白表达可作为临床肺癌早期诊断和患者预后判断的一个重要参考指标
355 lung specimens, including 10 normal tissues, 11 simple bronchial epithelial hyperplasias, 15 atypical hyperplasias, 13 in situ carcinomas, and 306 invasive carcinomas. Immunohistochemistry with anti-p53 monoclonal antibody Do-7. S-P staining study. The results showed that: 1 p53 protein staining was negative in normal bronchial epithelial and simple hyperplasia epithelium; 2 p53 protein overexpression was found in 40.0% (6/15) dysplasia, 46.2% (6/13) in situ Cancer and 52.9% (162/306) of invasive carcinoma; 3p53-positive tissue proliferating cell nuclear antigen (PCNA) frequency was significantly higher than p53-negative tissue; 4p53 protein overexpression was positively correlated with clinical staging and negatively correlated with survival time However, it has nothing to do with the histological type or grade of lung cancer. It is suggested that the expression of p53 gene protein can be used as an important reference index for early diagnosis of lung cancer and prognosis of patients.