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目的 了解高效抗逆转录病毒治疗后中国HIV/ AIDS患者淋巴细胞活化及CCR5、CXCR4表达的变化,探讨HIV感染者对于抗病毒治疗的免疫应答。方法 10例HIV /AIDS患者给予高效抗逆转录病毒治疗(HAART),用流式细胞仪检测治疗前和治疗第3、6 个月T淋巴细胞活化(HLA DR、CD38表达)及第二受体CCR5、CXCR4表达情况,比较HIV/ AIDS患者治疗前后淋巴细胞活化、第二受体表达的变化。结果 治疗前,10例HIV /AIDS患者CD4+、CD8+ T淋巴细胞活化水平均明显高于健康对照,CD8+ T淋巴细胞表面CCR5的表达明显高于健康对照,CXCR4 的表达明显低于健康对照(P<0.05);HAART治疗后,患者淋巴细胞活化水平随治疗时间明显下降(P<0.05),CD8+ T淋巴细胞表面CCR5表达水平显著降低(P< 0. 01), CXCR4 的表达升高;治疗6 个月时, CD38 CD4、HLA DRCD38 CD4、CCR5 CD8、CXCR4 CD8表达水平恢复至健康人水平;HIV AIDS淋巴细胞活化水平及第二受体CCR5的表达降低与HAART治疗后CD4+T淋巴细胞数量的升高具有显著的相关性。结论HAART能够降低中国HIV /AIDS患者淋巴细胞活化水平,使第二受体表达水平趋于正常,促进免疫功能的恢复。
Objective To investigate the changes of lymphocyte activation and the expression of CCR5 and CXCR4 in Chinese HIV / AIDS patients after effective antiretroviral therapy and to explore the immune response to HIV infection in HIV-infected patients. Methods Ten cases of HIV / AIDS patients were given high effective antiretroviral therapy (HAART), and the activation of T lymphocytes (HLA DR and CD38) and the second receptor CCR5, CXCR4 expression in patients with HIV / AIDS before and after treatment of lymphocyte activation, the expression of the second receptor changes. Results Before treatment, the activation levels of CD4 + and CD8 + T lymphocytes in 10 HIV / AIDS patients were significantly higher than those in healthy controls, the expression of CCR5 on CD8 + T lymphocytes was significantly higher than that in healthy controls, and the expression of CXCR4 was significantly lower than that in healthy controls (P < 0.05). After HAART treatment, the activation level of lymphocyte in patients decreased significantly (P <0.05), the expression of CCR5 on CD8 + T lymphocytes decreased significantly (P <0.01), and the expression of CXCR4 increased Month, CD38 CD4, HLA DRCD38 CD4, CCR5 CD8, CXCR4 CD8 expression levels returned to healthy levels; HIV AIDS lymphocyte activation level and the second receptor CCR5 expression decreased with the number of CD4 + T lymphocytes after HAART rise High has significant correlation. Conclusions HAART can reduce the level of lymphocyte activation in HIV / AIDS patients in China and make the expression level of the second receptor normal and promote the recovery of immune function.