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构建cDNA文库是发掘基因和研究基因功能的重要工具,也是基因组学研究的重要技术手段。棉花作为世界上重要的经济作物之一,研究人员已经构建了大量的棉花cDNA文库,为棉花基因发掘和功能研究积累了丰富的Expressed Sequence Tags(ESTs)资源。本文对近几年来cDNA文库在棉花基因发掘中的应用进行了总结。
The construction of cDNA library is an important tool to discover genes and to study the function of genes. It is also an important technical means of genomics research. As one of the most important cash crops in the world, researchers have constructed a large number of cotton cDNA libraries and have accumulated rich resources of Expressed Sequence Tags (ESTs) for cotton gene discovery and function research. This paper summarizes the application of cDNA library in cotton gene discovery in recent years.