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观察了小鼠接种高分泌ＩＬ－４的黑色素瘤细胞后体内免疫效应细胞（包括ＣＴＬ、ＮＫ细胞及ＬＡＫ细胞）抗肿瘤活性及其分泌的细胞因子（包括ＩＬ－２、ＩＦＮ－ｒ、ＴＮＦ及ＧＭ－ＣＳＦ）水平的变化，荷瘤后第１５天小鼠脾细胞ＮＫ活性及经诱导后的ＬＡＫ活性有所降低，而经诱导后的ＣＴＬ杀伤活性显著升高，荷瘤小鼠腹腔内巨噬细胞杀伤活性及其分泌的ＩＬ－１水平也明显升高；在荷瘤后第４天及第１２天出现两个高峰，实验结果表明，高分泌ＩＬ－４的黑色素瘤细胞体内生长受抑制是因为ＩＬ－４基因的导入及ＩＬ－４的分泌使体内ＣＴＬ及巨噬细胞杀伤活性提高，因而使机体抗肿瘤免疫功能得以增强。
The anti-tumor activity of cytokines (including CTL, NK cells, and LAK cells) and their secreted cytokines (including IL-2, IFN-r, and TNF) were observed after inoculation of melanoma cells with high secretion of IL-4 in mice. The level of GM-CSF was changed. The NK activity of spleen cells and the activity of LAK after induction were decreased on the 15th day after tumor-bearing. The cytotoxicity of CTL after induction was significantly increased, and the intratumoral giant tumor-bearing mice were significantly increased. The phagocytic cytotoxic activity and the secretion of IL-1 were also significantly increased; there were two peaks on the 4th and the 12th day after tumor-bearing. The experimental results showed that the melanoma cells with high secretion of IL-4 were inhibited in vivo growth. This is because the introduction of IL-4 gene and the secretion of IL-4 increase the killing activity of CTL and macrophage in vivo, thus enhancing the body’s anti-tumor immune function.