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目的 研究不同表面活性剂制备的硬脂酸纳米粒混悬液的表面性质与体外细胞摄取之间的关系。方法 以不同的表面活性剂采用乳化蒸发 低温固化法制备硬脂酸纳米粒混悬液并测定其表面接触角和表面张力 ,以小鼠腹腔巨噬细胞为细胞模型作体外细胞吞噬实验。结果 用苄泽类Brij 78[聚氧乙烯 (2 0 )硬脂酸醚 ],Myrj 5 3[聚氧乙烯 (5 0 )硬脂酸酯 ],Myrj 5 9[聚氧乙烯 (10 0 )硬脂酸酯 ]和PluronicF6 8[聚氧乙烯和聚氧丙烯嵌段聚合物 ,普郎尼克 188]为表面活性剂制备了粒径分别为 (16 2 .0± 6 7.4) ,(5 0 .2± 2 8.9) ,(32 6 .8± 195 .2 )和 (2 0 4.5± 91.3)nm的硬脂酸纳米粒。体外细胞摄取实验证明 ,各种纳米粒相对于硬脂酸溶液均可增加巨噬细胞对硬脂酸的摄取 ,其中以Myrj 5 9组摄取量最少 ;硬脂酸纳米粒表面的亲水性与巨噬细胞对纳米粒的摄取之间存在着明显的相关关系。结论 硬脂酸纳米粒溶液的表面性质可以影响纳米粒对巨噬细胞的趋向性
Objective To study the relationship between surface properties of stearic acid nanoparticles suspension prepared by different surfactants and in vitro cellular uptake. Methods Different surfactants were used to prepare stearic acid nanoparticles suspension by emulsion evaporation and the surface contact angle and surface tension were measured. The mouse peritoneal macrophages were used as the cell model for in vitro phagocytosis. Results Beryllilas Brij 78 [polyoxyethylene (20) stearate ether], Myrj 5 3 [polyoxyethylene (50) stearate], Myrj 5 9 [polyoxyethylene (16 2 .0 ± 6 7.4), (5 0. 2) and Pluronic F 6 8 [polyoxyethylene and polyoxypropylene block polymers, Pluronic 188] ± 2 8.9), (32 6 .8 ± 195 .2) and (2 0 4.5 ± 91.3) nm stearic acid nanoparticles. In vitro cell uptake experiments showed that various nanoparticles relative to the stearic acid solution can increase the macrophage uptake of stearic acid, of which Myrj 5 9 group intake the least; stearic acid nanoparticles surface hydrophilicity and There is a clear correlation between macrophage uptake of nanoparticles. Conclusion The surface properties of stearic acid nanoparticle solution can affect the tendency of nanoparticles to macrophages