论文部分内容阅读
为了挖掘可用于小麦茉莉酸调控通路及其穗部或花器官发育研究的候选基因,基于已发布的粗山羊草全基因组数据,利用生物信息学分析方法鉴定了粗山羊草JAZ基因家族,并从染色体分布、基因及蛋白结构、启动子顺式作用元件、系统进化关系和基因表达模式等方面对该基因家族的特征进行了比较分析。结果表明,本研究共鉴定到9个粗山羊JAZ基因,命名为AetJAZ1-AetJAZ9。染色体定位结果显示,9个粗山羊草JAZ基因分别定位在2D、5D、6D和7D染色体上,其中在7D染色体上分布最多。进化分析表明,粗山羊草JAZ蛋白与短柄草JAZ蛋白亲缘关系最近,且9个粗山羊草JAZ蛋白被分别聚类到了S5、S12、S13、S16和S20五个亚族中,其中S20亚族有五个粗山羊草JAZ蛋白。启动子分析表明,9个粗山羊草JAZ基因启动子顺式作用元件种类和数量存在差异,推测其各自的功能也存在差异。表达谱分析表明,粗山羊草JAZ基因在粗山羊草不同组织间存在不同的表达特性,且不存在组成型表达基因,其中,AetJAZ1和AetJAZ2表现出明显的组织特异性,分别只在穗部、雌蕊和雄蕊中特异表达。
In order to explore the candidate genes that could be used for the regulation of jasmonic acid regulation in wheat and their spike or floral organ development, the JAZ gene family of Aegilops tauschii was identified by bioinformatics analysis based on published whole genome data of Aegilops tauschii. Chromosome distribution, gene and protein structure, promoter cis-acting elements, phylogenetic relationships and gene expression patterns and other aspects of the gene family characteristics were compared. The results showed that JAZ gene of nine goats was identified and named AetJAZ1-AetJAZ9. Chromosomal mapping showed that JAZ genes of 9 Aegilops species were mapped on 2D, 5D, 6D and 7D chromosomes, respectively. Among them, JAZ gene was most distributed on chromosome 7D. Phylogenetic analysis showed that the JAZ protein of Aegilops taiga was closest to the JAZ protein of Brachiopsis, and nine JAZ proteins of Aegilops colchicus were clustered into five subfamilies of S5, S12, S13, S16 and S20, among which S20 Family has five goat grass JAZ protein. Promoter analysis showed that there are differences in the types and amounts of cis-acting elements of JAZ gene promoter in nine Aegilops tauschii, suggesting that their respective functions are also different. Expression profiling showed that the JAZ gene of Aegilops tauschii had different expression characteristics among different tissues of Aegilops tauschii and there was no constitutively expressed gene. Among them, AetJAZ1 and AetJAZ2 showed obvious tissue specificity, Pistil and stamens specifically expressed.