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荧光素异硫氰酸酯标记高密度脂蛋白－２保持天然高密度脂蛋白－２的生物活性。人肝癌ＳＭＭＣ－７７２１细胞与荧光标记无载脂蛋白Ｅ－高密度脂蛋白－２在３７℃培养３ｈ左右，细胞结合与内吞的荧光强度分别为１３．５±０．８和９．２±０．５（±ｓ土）。细胞进一步在３７℃培养２ｈ细胞释放的三氯醋酸沉淀和三氯醋酸可溶性荧光强度分别为５．１±０．４和０．６７±０．１７．４℃培养２ｈ，细胞释放三氯醋酸沉淀荧光强度为０．４１±０．１６。结果提示：①细胞内吞高密度脂蛋白－２没有经过溶酶体分解途径；②细胞释放高密度脂蛋白－２载脂蛋白是一种取决于温度的逆向胞饮机制。
The fluorescein isothiocyanate-labeled high-density lipoprotein-2 maintains the biological activity of natural high-density lipoprotein-2. Human hepatocarcinoma SMMC-7721 cells and fluorescence-labeled apolipoprotein E-high-density lipoprotein-2 were cultured at 37°C for 3 h, and the fluorescence intensities of cell binding and endocytosis were 13.5±0.8 and 9.2±, respectively. 0.5 (±s soil). The cells were further incubated at 37 °C for 2 h. The trichloroacetic acid precipitated by the cells and the soluble fluorescence intensity of trichloroacetic acid were 5.1 ± 0.4 and 0.67 ± 0.17.4 °C, respectively. The cells were incubated for 2 h and the cells released trichloroacetic acid. The fluorescence intensity was 0.41±0.16. The results suggest that: 1 Endocytosis of high-density lipoprotein-2 has not gone through the lysosomal pathway; 2 The release of high-density lipoprotein-2 apolipoprotein is a temperature-dependent reverse pinocytosis mechanism.