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1990~1993年对江苏蔬菜、花卉、中药材、林木和杂草疫病进行了采集调查和鉴定。本文报道江苏省18种由疫霉属真菌(Phytophthora)引起的植物新病害及病原菌鉴定结果。 病原菌的分离均采用选择性培养基,其配方为在每1000ml利马豆培养基中加入利福平100mg、青霉素、五氯硝基苯、苯莱特各50mg。孢子囊的诱导系将供试菌株在10%V8澄清培养液中培养3天后将获得的菌丝丛移入灭菌自来水中,每12h换水一次,直至产生孢子囊。异宗配合疫霉种卵孢子的产生系将供试
From 1990 to 1993, the investigation and identification of vegetables, flowers, Chinese herbal medicines, trees and weeds in Jiangsu were conducted. This paper reports 18 new plant diseases caused by Phytophthora and the identification of pathogenic bacteria in Jiangsu Province. Separation of pathogens are used selective medium, the formula is in every 1000ml limaidu medium added rifampicin 100mg, penicillin, pentachloronitrobenzene, benzene Wright each 50mg. Sporocysts Induction Line After the test strains were cultured in 10% V8 clarified medium for 3 days, the obtained mycelial clumps were moved into sterile tap water and the water was changed every 12 h until sporangial sacs were generated. Heterophile with Phytophthora sporulation production line will test