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以新分离的两种不同亚型CPV为毒种制备浓缩灭活苗和未浓缩灭活苗,通过与商品化弱毒苗的比较评价灭活疫苗的免疫效果。将CPV-S5(New CPV-2a)与CPV-1401(New CPV-2b)毒株扩大培养后浓缩灭活,制备了两种浓缩与两种未浓缩水溶剂灭活疫苗;将灭活疫苗和商品化二联弱毒苗分别接种5周龄HI阴性幼犬,分别在免疫后7 d、14 d、21 d、28 d测定HI抗体效价;首免36 d后取其中4只犬二免,其他犬进行攻毒。结果显示,各免疫组在第7天HI均高于1∶80,浓缩组显著高于未浓缩组,初步表明CPV灭活苗效果与抗原的浓度有直接的关系;灭活疫苗组与商品化弱毒苗组均差异显著;免疫组的犬均存活,而HI阴性犬死亡,经检测死亡犬CPV为阳性,表明制备的CPV灭活苗产生的特异性免疫应答均能抵抗强毒的攻击,对犬起到良好的免疫保护;二免后其HI抗体效价极显著提高,有效抗体能维持5个月,更有效的对免疫犬群进行保护。
The newly isolated two different subtypes of CPV were used as poisons to prepare concentrated inactivated and non-concentrated inactivated vaccine, and compared with commercial attenuated vaccine to evaluate the immune effect of inactivated vaccine. After inoculation of CPV-S5 (New CPV-2a) and CPV-1401 (New CPV-2b) strains, the two kinds of concentrated and two non-concentrated water-solvent inactivated vaccines were prepared. Commercially available attenuated dual vaccines were inoculated with 5-week-old HI negative puppies respectively HI HI antibody titer at 7 d, 14 d, 21 d, 28 d after immunization; Other dogs to attack. The results showed that the HI in each immunization group was higher than 1:80 on the 7th day, and the concentration in the concentrated group was significantly higher than that in the non-concentrated group, indicating that the effect of the CPV inactivated vaccine was directly related to the concentration of the antigen. The inactivated vaccine group was commercialized The attenuated vaccine group were significantly different; dogs in the immunized group survived, but the HI-negative dogs died, CPV was positive in the tested dogs, indicating that the specific immune response produced by CPV inactivated vaccine can resist the virulent challenge, Dogs play a good immune protection; two after the HI antibody titer was significantly improved, the effective antibody can be maintained for 5 months, more effective protection of the immune dog population.