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目的研究肝细胞癌(HCC)组织中C-JUN激活域连接蛋白1(JAB1)的表达及与P27kip1(P27)间的关系,探讨JAB1与临床病理及预后的关系。方法免疫组化检测HCC及相应癌旁肝组织中JAB1和P27的表达。Western blot及免疫沉淀检测JAB1和P27的表达及相互作用。结果HCC中,JAB1的表达高于癌旁肝组织,P27表达低于癌旁组织。JAB1的表达与组织分化程度、甲胎蛋白(AFP)值及转移相关(P<0.05)。HCC中JAB1与P27的表达呈负相关(r=-0.7077,P<0.001),并且能免疫共沉淀。结论JAB1的表达与P27负相关,JAB1作为P27的负性调控因子,可能与HCC的恶性进展及预后相关。
Objective To investigate the expression of C-JUN activated domain 1 (JAB1) and its relationship with P27kip1 (P27) in hepatocellular carcinoma (HCC) and to explore the relationship between JAB1 and clinical pathology and prognosis. Methods Immunohistochemistry was used to detect the expression of JAB1 and P27 in HCC and adjacent noncancerous liver tissues. Western blot and immunoprecipitation were used to detect the expression and interaction of JAB1 and P27. Results The expression of JAB1 in HCC was higher than that in paracancerous liver tissue, while the expression of P27 was lower than that in paracancerous tissues. The expression of JAB1 was correlated with the degree of tissue differentiation, AFP value and metastasis (P <0.05). The expression of JAB1 and P27 in HCC was negatively correlated (r = -0.7077, P <0.001), and co-immunoprecipitation. Conclusions The expression of JAB1 is negatively correlated with P27. JAB1, as a negative regulator of P27, may be related to the malignant progression and prognosis of HCC.