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目的利用原代培养的心房肌细胞建立快速起搏模型,研究L-型钙通道及钾通道Kv4·3在快速起搏早期的表达变化。方法原代培养大鼠心房肌细胞,并建立快速起搏细胞模型,利用RT-PCR以及Western-blot方法检测L-型钙通道α1c及钾通道Kv4·3在快速起搏3、6、12、24h后mRNA和蛋白的表达变化。结果快速起搏6h后L-型钙通道α1c的mRNA和蛋白表达较起搏前持续降低,并于24h时达到最低值;而钾通道Kv4·3mRNA和蛋白的表达在快速起搏12h后降低,并且在其后保持相对稳定的水平。结论快速起搏早期,原代培养心房肌细胞L-型钙通道α1c及钾通道Kv4·3的mRNA和蛋白表达均出现不同程度的降低,提示其发生了离子通道重构,并且可能是电重构的分子基础。
OBJECTIVE: To establish a rapid pacing model using primary cultured atrial myocytes to study the changes of L-type calcium channel and potassium channel Kv4.3 in early rapid pacing. Methods Primary rat atrial myocytes were cultured and fast paced cell models were established. The expression of L-type calcium channel α1c and potassium channel Kv4 · 3 in rapid paced 3, 6, 12 were detected by RT-PCR and Western- 24h after mRNA and protein expression changes. Results The mRNA and protein expression of L-type calcium channel α1c decreased rapidly after pacing for 6 h and reached the lowest value at 24 h. However, the expression of Kv4 · 3 mRNA and protein in K + channel decreased after 12 h of rapid pacing, And thereafter maintain a relatively stable level. Conclusion In the early stage of rapid pacing, mRNA and protein expression of L-type calcium channel α1c and potassium channel Kv4 · 3 in primary cultured atrial myocytes decreased to different extents, suggesting that ion channel remodeling occurred, The molecular basis of structure.