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为了探索SWEET基因家族在棉花中的分布和作用,基于二倍体D基因组雷蒙德氏棉基因组数据,利用生物信息学方法对SWEET基因家族完成了全基因组水平的鉴定,并进行了系统发育、基因结构、染色体定位和跨膜结构域分析。共获得31个雷蒙德氏棉SWEET基因,分散或成簇分布在11条染色体上,分为3个不同功能的亚家族;跨膜结构域预测显示大部分雷蒙德氏棉SWEET基因含7个跨膜结构域,少部分含6或7个跨膜结构域;基因结构分析显示除GrSWEET6、GrSWEET7、GrSWEET9、GrSWEET19含4个内含子外,其余27个SWEET基因均含有5个内含子。这些结果将为进一步深入研究SWEET基因在棉花生长发育中功能奠定坚实的数据基础。
In order to explore the distribution and function of SWEET gene family in cotton, based on the diploid D genomic DNA of the genome of Raymond Cotton, genome-wide identification of the SWEET gene family was accomplished by using bioinformatics methods and phylogeny was carried out. Gene structure, chromosomal location and transmembrane domain analysis. A total of 31 Raman cotton SWEET genes were obtained and distributed on 11 chromosomes in clusters and divided into three subfamilies with different functions. The transmembrane domain prediction showed that most of the SWEET genes in the two cultivars were 7 Transmembrane domain, a small part of which contains 6 or 7 transmembrane domains; gene structure analysis showed that in addition to GrSWEET6, GrSWEET7, GrSWEET9, GrSWEET19 contains four introns, the other 27 SWEET genes contain 5 introns . These results will lay a solid foundation for further study on the function of SWEET gene in cotton growth and development.