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采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、开放ODS柱色谱以及HPLC等对海洋来源真菌Hypocreavirens的菌丝体进行化学成分分离,通过理化性质和波谱数据鉴定化合物的结构。从菌丝体的丙酮提取物中分离得到8个化合物,分别鉴定为1(10→6)Abeo-(22E,24R)-ergosta-5,7,9,22-tetraene-3α,11α-diol(Ⅰ)、24-甲烯基羊毛脂烷-8-烯-3β-醇(Ⅱ)、(22E,24R)-麦角甾-5,7,22-三烯-3β-醇(Ⅲ)、(22E,24R)-麦角甾-8,22-二烯-3β,5α,6β,7α-四醇(Ⅳ)、5α,9α-过氧-(22E,24R)-麦角甾-7,22-二烯-3β,6β-二醇(Ⅴ)、5α,8α-过氧-(22E,24R)-麦角甾-6,22-二烯-3β-醇(Ⅵ)、(22E,24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇(Ⅶ)、3β,5α,9α-三羟基-(22E,24R)-麦角甾-7,22-二烯-6-酮(Ⅷ),其中化合物Ⅰ、Ⅱ、Ⅳ、Ⅴ、Ⅷ均为首次从该属真菌中分离得到,化合物Ⅲ、Ⅵ、Ⅶ为首次从该种真菌中分离得到。
The mycelia of marine fungi Hypocreavirens were separated by silica gel column chromatography, Sephadex LH-20 column chromatography, ODS column chromatography and HPLC. The structures of the compounds were identified by their physico-chemical properties and spectral data. Eight compounds were isolated from the mycelium acetone extract and identified as 1 (10 → 6) Abeo- (22E, 24R) -ergosta-5,7,9,22-tetraene-3α, 11α-diol (II), (22E, 24R) -ergosta-5,7,22-trien-3β-ol , 24R) -ergosta-8,22-diene-3β, 5α, 6β, 7α-tetraol (IV), 5α, 9α -peroxy- (22E, 24R) -ergosta-7,22-diene (22E, 24R) -ergosta-6,22-dien-3β-ol (Ⅵ), (22E, 24R) 7,22-diene-3β, 5α, 6β-triol (VII), 3β, 5α, 9α-trihydroxy- (22E, 24R) Among them, compounds Ⅰ, Ⅱ, Ⅳ, Ⅴ and Ⅷ were isolated from this genus for the first time. Compounds Ⅲ, Ⅵ and Ⅶ were isolated from this fungus for the first time.