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本试验结果表明，Ａ蛋白夹心酶联法检测苹果花叶病毒，适宜工作浓度第１抗血清为１／１０３－１／１０４，第２抗血清为１／１０３。双抗体夹心法检测该病毒，抗体ＩｇＧ浓度愈高，阳性样品与阴性样品吸光值之比愈大，当ＩｇＧ稀释４００倍时，两者的吸光值无明显差别。取休眠期枝条进行检测，以含０．０５％吐温－２０的磷酸缓冲盐水（ＰＢＳＴ）十２％聚乙烯吡咯烷酮十０．１％卵蛋白作提取缓冲液获得了较好的检测结果，添加铜试剂能明显提高阳性样品吸光值。春季嫩叶的检测效果最佳。
The results showed that A protein sandwich enzyme-linked detection of apple mosaic virus, the appropriate working concentration of the first antiserum was 1 / 103-1 / 104, the second antiserum was 1/103. Double antibody sandwich method for the detection of the virus, the higher the concentration of IgG antibody, positive samples and negative samples the greater the ratio of absorbance, IgG diluted 400 times, the two absorbance no significant difference. Take the dormant branch to detect, with 0.05% Tween-20 phosphate buffered saline (PBST) ten 2% polyvinylpyrrolidone ten 0.1% egg protein extraction buffer to obtain a better test results, add Copper reagents can significantly increase the absorbance of positive samples. The detection of young leaves best.