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目的:探讨三维打印β-磷酸三钙(β-Tricalcium Phosphate,β-TCP)颌骨修复支架的生物学特性及体内成骨作用。方法:采用自动注浆技术制作β-TCP支架,将前成骨细胞(MC35T3-E1)接种在支架上,扫描电镜(SEM)观察材料结构与细胞黏附,CCK-8法检测细胞增殖,ALP法检测碱性磷酸酶活性。将2种支架复合重组人骨形成蛋白-2(recombinant human bone morphogenetic protein-2,rhBMP-2)后植入大鼠体内,发泡法制作的β-TCP支架为对照组,6周后取材行组织学观察。结果:三维打印支架具有规则多孔的立体结构,适合细胞黏附,且增殖及分化能力均高于对照组(P<0.05)。组织学显示复合rhBMP-2后三维打印支架新骨生成量高于发泡法制作的β-TCP支架(P<0.05)。结论:三维打印TCP支架生物相容性良好,复合rhBMP-2后可异位成骨。
Objective: To investigate the biological characteristics and in vivo osteogenesis of three-dimensionally printed β-Tricalcium Phosphate (β-TCP) mandibular restoration. Methods: β-TCP scaffolds were made by using automatic grouting technique. Pre-osteoblasts (MC35T3-E1) were seeded on scaffolds. The structure and cell adhesion were observed by scanning electron microscopy (SEM) Alkaline phosphatase activity was measured. The two kinds of scaffolds were injected into rhBMP-2 by recombinant human bone morphogenetic protein-2 (rhBMP-2). The β-TCP scaffold made by foaming method was used as the control group. After 6 weeks, Learn to observe. Results: The three-dimensional scaffolds had a regular porous structure and were suitable for cell adhesion. The ability of proliferation and differentiation of three-dimensional scaffolds was higher than that of the control group (P <0.05). Histology showed that the new bone formation rate of the three-dimensional printing stent was higher than that of the β-TCP stent made by the foaming method (P <0.05). Conclusion: The biocompatibility of 3D printed TCP scaffolds is good, and rhBMP-2 can ectopic osteogenesis.