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用革兰染色和免疫组化染色技术，对１２９例鼻咽癌、８５例喉癌、２０例鼻咽慢性炎、２０例声带息肉进行细菌Ｌ型检测，同时对Ｌ型阳性和阴性组织的Ｐ５３、ＣｅｒｂＢ－２表达进行对比分析。结果显示，鼻咽癌、喉癌的革兰染色Ｌ型检出率分别为７８３％和７６５％。肿瘤组的Ｐ５３、ＣｅｒｂＢ－２表达率明显高于炎症组（Ｐ＜０００５）。鼻咽癌、喉癌中Ｌ型阳性组的Ｐ５３、ＣｅｒｂＢ－２表达率也高于其Ｌ型阴性组（Ｐ＜０００５）。表明Ｌ型感染与鼻咽癌、喉癌及Ｐ５３、ＣｅｒｂＢ－２过表达有关。提示Ｌ型参与了Ｐ５３、ＣｅｒｂＢ－２基因突变，并可能在鼻咽癌、喉癌发生中起作用。
Gram stain and immunohistochemical staining technique were used to detect L type of bacteria in 129 cases of nasopharyngeal carcinoma, 85 cases of laryngeal carcinoma, 20 cases of chronic nasopharyngitis and 20 cases of vocal cord polyp. Meanwhile, the positive rate of P53 , CerbB-2 expression for comparative analysis. The results showed that the detection rates of gram-positive L-type of nasopharyngeal carcinoma and laryngeal carcinoma were 783% and 765% respectively. The expression of P53 and CerbB-2 in the tumor group was significantly higher than that in the inflammation group (P <0005). The positive rates of P53 and CerbB-2 in L-positive group were also higher than those in L-negative group (P <0005). L type infection and nasopharyngeal carcinoma, laryngeal cancer and P53, CerbB-2 overexpression. It suggests that L-type is involved in the mutation of P53 and CerbB-2 genes and may play a role in the carcinogenesis of nasopharyngeal carcinoma and laryngeal carcinoma.