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作者对16例苔癣样糠疹(PL)患者进行了研究:取其新鲜无鳞屑的紫癜性丘疹损害作活组织检查,用绵羊抗人IgG、IgM和IgA及兔抗人C_3的最适稀释的荧光素标记的结合物染5微米厚的切片,以荧光显微镜作直接免疫荧光(IF)检查。3例患者的未受累的皮肤及16名健康对照者的正常皮肤用同样的方法进行检查。此外,还从PL活动期和非活动期的患者取血分离血清,加入聚乙二醇(PEG)使血清中可溶性免疫复合物沉淀以检测循环免疫复合物。用单向放射免疫扩散法测定复合物中C_(1q)和
The authors studied 16 patients with lichen pityriasis pleuropneumonia (PL): freshly scaly purpura-free papulosis for biopsy, sheep anti-human IgG, IgM and IgA and rabbit anti-human C_3 optimal dilution Fluorescein-labeled conjugates were stained with 5-μm-thick sections and examined by fluorescence microscopy for direct immunofluorescence (IF). The normal skin of 3 patients with uninvolved skin and 16 healthy controls was examined in the same manner. In addition, sera from patients with active and inactive PL were also separated from serum, and polyethylene glycol (PEG) was added to precipitate soluble immune complexes in serum to detect circulating immune complexes. The C_ (1q) and C_ (1q) in the complex were determined by one - way radioimmunoassay