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目的 LuxS基因是变形链球菌生物膜早期形成过程中的关键基因,构建该基因的缺陷菌。方法采用长臂同源多聚酶链反应(LFH-PCR)方法构建含红霉素耐药基因片段的LuxS基因上、下游同源序列的连接片段,转化到变形链球菌中,在红霉素的平板上筛选缺陷菌株,并采用PCR鉴定。结果对变形链球菌LuxS基因缺陷菌株进行PCR和DNA序列测定分析证实构建成功。结论成功构建出变形链球菌LuxS基因的缺陷菌株,为后期针对变形链球菌LuxS基因的相关研究奠定基础。
Objective LuxS gene is a key gene during the early formation of Streptococcus mutans biofilm and constructs the defective gene of this gene. Methods Long-arm homologous polymerase chain reaction (LFH-PCR) was used to construct the upstream and downstream homologous sequences of the LuxS gene fragment containing erythromycin resistance gene and transformed into Streptococcus mutans. The defective strains were screened and identified by PCR. Results PCR and DNA sequence analysis of LuxS gene-deficient strains of Streptococcus mutans confirmed the successful construction. Conclusion The defective strain of Streptococcus mutans LuxS gene was constructed successfully, which laid the foundation for the related research of LuxS gene of Streptococcus mutans.