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[Objective] The research was aimed at identifying the optimum culture medium for the proliferation culture of Atractylodes lancea(Thunb.) DC.subsp.luotianensis S.L.Hu et X.F.Feng,so as to provide a technical support for the industrialized production of Atractylodes lancea(Thunb.) DC.subsp.luotianensis S.L.Hu et X.F.Feng seedlings.[Method] The optimum basic culture medium was screened out by employing tender branch as explants,and the proportion of 6-BA,KT,NAA and IBA was optimized by L9(34) orthogonal test.[Result] The optimum basic culture medium was N68,and the optimum combination of plant growth regulators was 1.0 mg/L 6-BA,1.0 mg/L KT,0.5 mg/L NAA and 0.2 mg/L IBA.[Conclusion] The optimum medium for the proliferation culture was obtained in this study,which provided a technical support for the industrialized production of Atractylodes lancea(Thunb.) DC.subsp.luotianensis S.L.Hu et X.F.Feng seedlings.
[Objective] The research was aimed at identifying the optimum culture medium for the proliferation culture of Atractylodes lancea (Thunb.) DC.subsp.luotianensis SLHu et XFFeng, so as to provide a technical support for the industrialized production of Atractylodes lancea Thunb.) DC.subsp.luotianensis SLHu et XFFeng seedlings. [Method] The optimum basic culture medium was screened out by employing tender tender branch as explants, and the proportion of 6-BA, KT, NAA and IBA was optimized by L9 (34) orthogonal test. [Result] The optimum basic culture medium was N68, and the optimum combination of plant growth regulators was 1.0 mg / L 6-BA, 1.0 mg / L KT, 0.5 mg / L NAA and 0.2 mg / L IBA. [Conclusion] The optimum medium for the proliferation culture was obtained in this study, which provided a technical support for the industrialized production of Atractylodes lancea (Thunb.) DC.subsp.luotianensis SLHu et XFFeng seedlings.