为了分析糖皮质激素对神经系统快速作用的机制,我们首先建立了大鼠海马脑片细胞内记录的技术,然后观察糖皮质激素对海马CA_1、CA_2区神经元细胞膜电位和输入阻抗的影响。实验结果表明;糖皮质激素灌流1～2min即可使一部分海马神经元超极化,同时伴有输入阻抗升高的趋势。这些现象提示:糖皮质激素可以非基因形式作用于细胞膜,作用机制可能在于其改变了膜静息电位。 To analyze the mechanism of glucocorticoid’s rapid action on the nervous system, we first established the intracellular recording technique in rat hippocampal slices and then observed the effect of glucocorticoid on the membrane potential and the input impedance of neurons in hippocampal CA1 and CA2 area. Experimental results show that: glucocorticoid perfusion 1 ~ 2min can make part of the hippocampal neurons hyperpolarized, accompanied by the trend of increased input impedance. These phenomena suggest that glucocorticoid can act on the cell membrane non-genetically, the mechanism may be that it changes the membrane resting potential.