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目的:探讨腺样体摘除术对腺样体肥大患儿淋巴细胞亚群的影响。方法:选取2014年12月至2015年8月份在东营市人民医院进行腺样体摘除术的腺样体肥大患儿50例作为研究对象,采用流式细胞术检测CD3~+、CD3~+CD4~+、CD3~+CD8~+及CD19~+的淋巴细胞亚群的百分比,并与正常儿童对照组比较,观察患儿各项指标的变化。结果:患儿术前及术后1个月CD3~+、CD4~+/CD8~+及CD19~+细胞百分比分别较对照组明显降低(t检验值分别为2.55,2.33,2.61,2.43,2.26,2.52,P<0.05),术后3个月时CD3~+、CD4~+/CD8~+及CD19~+细胞百分比较术前明显回升(t检验值分别为2.53,2.59,2.30,P<0.05),与对照组比较无明显改变(P>0.05)。结论:腺样体肥大患儿较正常儿童淋巴细胞亚群含量明显降低。腺样体摘除术后3个月淋巴细胞亚群含量明显回升,提示免疫功能恢复正常。
Objective: To investigate the effect of adenoid excision on lymphocyte subsets in children with adenoid hypertrophy. Methods: Fifty cases of adenoid hypertrophy in children underwent adenoidectomy from December 2014 to August 2015 in our hospital were enrolled in this study. Flow cytometry was used to detect CD3 +, CD3 + CD4 ~ +, CD3 ~ + CD8 ~ + and CD19 ~ + lymphocyte subsets, and compared with the normal children’s control group to observe the changes of various indicators in children. Results: The percentage of CD3 ~ +, CD4 ~ + / CD8 ~ + and CD19 ~ + cells were significantly lower than those of the control group before operation and one month after operation (t-test values were 2.55, 2.33, 2.61, 2.43 and 2.26 , 2.52, P <0.05). At 3 months after operation, the percentage of CD3 ~ +, CD4 ~ + / CD8 ~ + and CD19 ~ + cells increased significantly (t = 2.53,2.59,2.30, P < 0.05), no significant change compared with the control group (P> 0.05). Conclusion: The content of lymphocyte subsets in children with adenoid hypertrophy is significantly lower than that in normal children. Lymphocyte subsets recovered significantly at 3 months after adenoidctomy, suggesting that immune function returned to normal.