论文部分内容阅读
从沙蒿籽中提取出水溶性胶多糖,经柱色谱分离纯化得到一种中性多糖组分ASPI-A.采用高效凝胶渗透色谱法(HPGPC)测定其为均一性多糖,平均分子量为5.42×104Da.经IR,GC部分酸水解、甲基化分析等方法对该多糖的化学结构进行了表征.结果表明,该多糖由阿拉伯糖、甘露糖、葡萄糖及半乳糖组成,其物质的量的比为1∶2.8∶4.9∶1.9.ASPI-A为多分支结构,以(1→4)-β-Glc构成主链,部分葡萄糖C6存在分支,由甘露糖以-4)Man(1-连接在葡萄糖C6位,Glc(1-和Gal(1-连接在甘露糖C4位构成.免疫活性实验结果表明,ASPI-A在10～50μg·mL-1浓度范围内对ConA诱导的小鼠T淋巴细胞增殖反应具有促进作用.
ASPI-A, a neutral polysaccharide fraction, was isolated and purified from Artemisia annua L. A homogeneous polysaccharide was determined by high performance gel permeation chromatography (HPGPC) with an average molecular weight of 5.42 × 104Da.The chemical structure of the polysaccharide was characterized by IR, GC partial acid hydrolysis and methylation analysis.The results showed that the polysaccharide was composed of arabinose, mannose, glucose and galactose, the ratio of the amount of substance Is 1: 2.8: 4.9: 1.9. ASPI-A is a multi-branched structure with the main chain of (1 → 4) -β-Glc and partial glucose C6 is branched from mannose Glucose C6, Glc (1- and Gal (1-linked in the mannose C4 position.) Immunological activity results showed that ASPI-A in the concentration range of 10 ~ 50μg · mL-1 ConA induced mouse T lymphocytes Proliferative response has a promoting effect.