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为了探讨肿瘤坏死因子-α(TNF-α)对奶牛乳腺成纤维细胞增殖的影响,试验将经体外培养的奶牛乳腺成纤维细胞用不同度浓度的TNF-α作用,分别于不同时间加入3-(4,5-二甲基噻唑-2-YL)2,5-二苯基-四唑溴盐(MTT)溶液,应用MTT比色法检测细胞增殖情况。结果表明:体外培养的奶牛乳腺成纤维细胞随TNF-α浓度(0.1,1,5,10,50ng/mL)的升高,MTT比色法的平均光密度值(OD值)呈递增的趋势,与对照组相比差异显著(P<0.05),具有统计学意义。说明TNF-α具有促进奶牛乳腺成纤维细胞增殖的作用。
In order to investigate the effects of tumor necrosis factor-α (TNF-α) on the proliferation of mammary fibroblasts in vitro, the effects of different concentrations of TNF-α on mammary fibroblasts cultured in vitro were tested by adding 3- (4,5-dimethylthiazol-2-YL) 2,5-diphenyl-tetrazolium bromide (MTT) solution. MTT assay was used to detect cell proliferation. The results showed that with the increase of TNF-α concentration (0.1, 1, 5, 10, 50 ng / mL), the average optical density (OD) value of MTT colorimetric method showed an increasing trend , Compared with the control group, the difference was significant (P <0.05), with statistical significance. TNF-α can promote the proliferation of mammary fibroblasts.