论文部分内容阅读
采用营养液砂培方法,研究了不同浓度NaCl胁迫(0、50、100、150、200和250mmol·L-1)对“定莜6号”燕麦幼苗生长、活性氧代谢和渗透调节物质含量的影响。结果表明:NaCl胁迫显著抑制燕麦幼苗的生长,抑制程度随NaCl浓度提高而增强,燕麦可耐受的最高NaCl浓度约为150mmol·L-1;随着NaCl浓度的增加,叶片O2·-产生速率、H2O2和丙二醛含量明显增加,超氧化物歧化酶、过氧化物酶和抗坏血酸过氧化物酶活性先升后降,过氧化氢酶活性迅速下降后逐渐升高,NaCl胁迫明显降低了谷胱甘肽含量,而抗坏血酸含量变化不大;NaCl胁迫显著提高了叶片脯氨酸含量,Na+含量随着NaCl浓度增加不断提高,K+含量和K+/Na+逐渐下降,质膜H+-ATP酶活性、总可溶性蛋白、热稳定蛋白和热不稳定蛋白含量先升后降,游离氨基酸含量先降后升,可溶性糖含量呈降-升-降趋势变化;盐胁迫下活性氧代谢失调和Na+、K+平衡破坏及积累有机溶质进行渗透调节时更多能量的消耗可能是燕麦生长受抑的重要因素。
The effects of NaCl stress (0, 50, 100, 150, 200 and 250 mmol·L-1) on the growth of oat seedlings, the metabolism of active oxygen and osmoregulation substances Effect of content. The results showed that: NaCl stress significantly inhibited the growth of oat seedlings, the degree of inhibition increased with the increase of NaCl concentration, and the maximum tolerable NaCl concentration in oats was about 150 mmol·L-1. With the increase of NaCl concentration, the leaf O2 · - production rate , H2O2 and malondialdehyde content increased significantly, the activities of superoxide dismutase, peroxidase and ascorbate peroxidase increased first and then decreased, catalase activity decreased rapidly and then increased gradually, and NaCl stress reduced the valley obviously While the content of ascorbic acid did not change much. The proline content of leaves increased significantly under NaCl stress. The content of Na + increased with the increase of NaCl concentration, K + content and K + / Na + The contents of total soluble protein, thermostable protein and heat labile protein increased firstly and then decreased, the content of free amino acid decreased first and then increased, the soluble sugar content showed a decreasing-rising-decreasing trend; the activity of active oxygen metabolism and balance of Na + and K + under salt stress Destruction and accumulation of organic solutes osmotic adjustment more energy consumption may be an important factor inhibiting the growth of oats.