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目的:研究替莫唑胺复方脂质体的制备工艺,并评价其体外抗肿瘤作用。方法:采用薄膜分散法制备复方脂质体,对处方进行优化;同时对脂质体进行粒径、Zeta电位表征;并采用磺酰罗丹明B蛋白(SRB)法考察脂质体对C6胶质瘤细胞的抗增殖作用。结果:采用最优处方,制备的复方脂质体中替莫唑胺和表没食子儿茶素没食子酸酯的包封率分别为(20.87±0.22)%和(85.86±0.31)%;平均粒径和Zeta电位分别为(170.1±0.42)nm和(-8.22±0.66)mV;制备得到的复方脂质体对C6细胞具用较强的抑制作用。结论:本方法制备的脂质体方法简单,粒径均一,且体外抗肿瘤作用强。
Objective: To study the preparation technology of temozolomide compound liposomes and to evaluate the anti-tumor effect in vitro. Methods: The liposomes were prepared by thin-film dispersion method and the prescriptions were optimized. Meanwhile, the liposomes were characterized by particle size and Zeta potential. The effects of liposome on C6 glial Anti-proliferative effects of tumor cells. Results: The entrapment efficiencies of temozolomide and epigallocatechin gallate in the prepared liposomes were (20.87 ± 0.22)% and (85.86 ± 0.31)%, respectively. The average particle size and Zeta potential (170.1 ± 0.42) nm and (-8.22 ± 0.66) mV, respectively. The prepared compound liposomes had strong inhibitory effect on C6 cells. Conclusion: The liposome prepared by this method has the advantages of simple method, uniform particle size and strong antitumor activity in vitro.