目的探讨饮水中微囊藻毒素(MC-LR)对小鼠糖脂代谢的影响及其可能的机制。方法将30只健康4周龄C57Bl/6纯系雄性小鼠按体重随机分为3组,分别为对照(自来水)组、低(40μg/L)、高(80μg/L)剂量MC-LR染毒组,每组10只。采用自由饮水方式持续染毒9个月。测定小鼠体重和腹腔脂肪组织重量,并计算脂肪脏器系数。测定糖脂水平及肝脏功能,并采用小鼠脂质动力学相关蛋白检测芯片试剂盒同时检测小鼠血清中与肥胖、代谢综合征相关蛋白的相对表达水平。结果与对照组相比,低、高剂量MC-LR染毒组小鼠的体重、腹腔脂肪重量及其脏器系数虽略有增加,但差异均无统计学意义;高剂量MC-LR染毒组小鼠血清谷氨酸氨基转移酶(ALT)水平和高、低剂量MC-LR染毒组血清天冬氨酸氨基转移酶(AST)、葡萄糖水平均较高,高、低剂量MC-LR染毒组血清甘油三酯(TG)水平较低,差异有统计学意义(P<0.05,P<0.01);而高、低剂量MC-LR染毒组血清胆固醇(TC)、高密度脂蛋白(HDL)水平无显著变化(P>0.05)。芯片结果显示,与新鲜自来水组比较,低剂量组上调表达≥1.5倍的蛋白有7个,高剂量组则达到17个。各因子具体功能分析结果表明,上调表达涉及最多的是脂质代谢和胰岛素抵抗相关的蛋白,炎症相关蛋白的表达水平也明显增加。结论饮用水MC-LR染毒可以引起小鼠糖脂代谢紊乱,脂质代谢、胰岛素抵抗和炎症反应可能在其中发挥了重要作用。 Objective To investigate the effect of microcystin (MC-LR) in drinking water on glucose and lipid metabolism in mice and its possible mechanism. Methods Thirty healthy C57Bl / 6 male mice of 4 weeks old were randomly divided into three groups according to body weight: control (water) group, low (40μg / L) and high (80μg / L) Toxic group, 10 in each group. Continuous drinking water for 9 months. The weight of mice and the weight of abdominal fat tissue were measured, and the fat organ coefficient was calculated. The level of glycolipid and the function of liver were measured. The relative expression level of protein related to obesity and metabolic syndrome in mouse serum was also detected by using the mouse lipid dynamics-related protein detection chip kit. Results Compared with the control group, the body weight, celiac fat weight and visceral fat mass of mice in low and high dose MC-LR groups increased slightly, but the difference was not statistically significant. High-dose MC-LR The levels of serum glutamate aminotransferase (ALT) and serum aspartate aminotransferase (AST) and glucose of high and low dose MC-LR groups were higher than those of high and low dose MC-LR The levels of serum triglyceride (TG) in the exposed group were lower than those in the control group (P <0.05, P <0.01), while the levels of serum cholesterol, high density lipoprotein (HDL) did not change significantly (P> 0.05). Chips results showed that compared with the fresh tap water group, the low-dose group up-regulated the expression of ≥1.5 times the protein 7, the high-dose group reached 17. The analysis of the specific function of each factor showed that up-regulated expression was the most important protein involved in lipid metabolism and insulin resistance, and the expression level of inflammatory-related protein was also significantly increased. Conclusion MC-LR drinking water can cause glucose and lipid metabolism disorder in mice, and lipid metabolism, insulin resistance and inflammatory reaction may play an important role.