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目的:建立大鼠离体肝动脉/门静脉灌流系统的实验方法。方法:雌性Wistar大鼠麻醉状态下,肝动脉、门静脉和肝静脉插管;经肝动脉冲净残留血液,灌流状态下游离肝脏;定量蠕动泵调节肝动脉或门静脉的Krebs-Henseleit平衡液(或加等渗葡聚糖)的灌流流速,泰盟BL-420S生物机能实验系统监测肝动脉或门静脉的压力变化,采用Prism-4非线型可变斜率回归获得“有或无胶体渗透压”KH液灌流的流速-压力曲线及方程式,计算半效流速及其95%的可信限。结果:无胶体渗透压灌流状态下的肝脏系数无差别,肝动脉的半效流速为2 217(95%CI 1 209～4 068)μl·min~(-1),对数流速-压力直线方程为Y=142.4X+706.9;门静脉半效流速为3 791(95%CI 3 549～4 049)μl·min~(-1),对数流速-压力直线方程为Y=479.6X+5 034。等效胶体渗透压灌流状态下的肝脏系数无差别,肝动脉半效流速为3 754(95%CI 3 175～4 440)μl·min~(-1),对数流速-压力直线方程为Y=133.5X-719.0;门静脉半效流速为6 018(95%CI 5 565～6 508)μl·min~(-1),对数流速-压力直线回归方程为Y=538.3X+4 704。半效流速接近正常大鼠生理平均值,各半效流速95%可信限涵盖大鼠正常生理状态的变化范围。有或无胶体渗透压灌流状态之间总体无差别。结论:在恒流测压模式下,等渗灌流大鼠离体肝动脉和门静脉灌流系统的视窗时程长、机能变化稳定、操作流程简便,为认识肝动脉和门静脉离体舒缩机制奠定了基础。
Objective: To establish an experimental method of isolated rat hepatic artery / portal vein perfusion system. Methods: The female Wistar rats were intubated under hepatic artery, portal vein and hepatic vein under anesthesia. The residual blood was rinsed through the hepatic artery and the free liver under perfusion. The peristaltic pump was used to regulate the Krebs-Henseleit balance fluid of the hepatic artery or portal vein The blood flow of perfusion was measured with the Prism-4 non-linear variable slope regression, and the blood pressure was measured by the Thai-BL-420S bio-functional experimental system to monitor the change of hepatic artery or portal vein pressure. "Flow-pressure curves and equations for KH liquid perfusion are used to calculate the half-flow rate and its 95% confidence limit. RESULTS: There was no difference in the hepatic coefficient between two groups. The half-effective velocity of the hepatic artery was 2 217 (95% CI 1 209 ~ 4 068) μl · min -1. The logarithmic velocity-pressure linear equation (Y = 142.4X + 706.9). The half-effective flow rate of portal vein was 3 791 (95% CI 3 549-4 049) μl · min -1. The logarithmic velocity-pressure linear equation was Y = 479.6X + 5034. The hepatic coefficient of hepatic arterial half-effect was 3 754 (95% CI 3 175-4 440) μl · min -1 with equivalent colloid osmotic pressure perfusion. The logarithmic velocity-pressure linear equation was Y = 133.5X-719.0. The half-effective flow rate of portal vein was 6 018 (95% CI 565-6 508) μl · min -1. The logarithmic velocity-pressure linear regression equation was Y = 538.3X + 4 704. The half-flow velocity approached the physiological average of normal rats, and the 95% confidence limits for each half-effect flow covered the range of changes in normal physiological conditions in rats. There was no difference between the groups with and without colloid osmotic pressure perfusion. CONCLUSION: Under constant current manometry mode, the window of ex vivo hepatic artery and portal vein perfusion system in isosected perfusion rats is long, the function changes steadily and the operation procedure is simple, which lays the foundation for understanding the mechanism of isolated and contractile contraction of hepatic artery and portal vein basis.